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Electrophoresis and DNA Fragment Length Determination
Configure & Price
IS.3300
In this activity students will perform agarose electrophoresis on three DNA samples that have already been treated with restriction enzymes. Students will not only learn about the process of DNA electrophoresis but learn the techniques associated with the process, such as setting up an agarose gel, loading DNA samples in the agarose gel, and staining the gel in order to visualize the DNA bands. They will also learn how to determine the sizes of unknown DNA fragments after examining their results. The kit includes specially-treated DNA samples that do not require refrigeration or freezing, prepared agarose that may simply be melted in a hot water bath or microwave, TBE electrophoresis buffer, and DNA stain.
Includes:
- 200mL 0.8% agarose
- 500mL 5X Tris Borate ETA Buffer
- 60mL 20X DNA Stain
- LAMBDA DNA ECORI DIGEST w/ loading dye
- LAMBDA DNA HINDIII DIGEST w/ loading dye
- LAMBDA DNA ECORI/HIND III DIGEST w/ loading dye
IS.3300
- Items needed, but not included
- Agarose electrophoresis chamber
Electrophoresis power supply
Micropipettes w/ 10μl micropipette tips
Gel staining trays
Microwave or hot water bath
Distilled or deionized water - Quantity
- 10 lab groups
- Includes
- Teachers manual and Student Study Guide copymasters
Resources